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KMID : 0354720070310050383
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Journal of Korean Diabetes Association
2007 Volume.31 No. 5 p.383 ~ p.390
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Cytoprotective Effect by Antioxidant Activity of Quercetin in INS-1 Cell Line
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Kwon Min-Jung
Jeong Hye-Sook
Kim Mi-Kyung
Kang Sung-Hoon
Seo Kwnag-Wook
Song Jae-Kwang
Yoon Tae-Yeon
Jeon Min-Kyung
Ha Tae-Hwan
Yoon Chang-Sin
Kim Mi-Kyung
Lee Woo-Je
Noh Jung-Hyun
Kwon Soo-Kyung
Kim Dong-Jun
Koh Kyung-Soo
Lee Byung-Doo
Lim Kyung-Ho
Lee Soon-Hee
Park Jeong-Hyun
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Abstract
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Background: Oxidative stress is induced under diabetic conditions and causes various forms of tissue damages in the patients with diabetes. Recently, pancreatic beta cells are regarded as a putative target of oxidative stress-induced tissue damage, and this seems to explain in part the progressive deterioration of beta cell function in type 2 diabetes. The aim of this study was to examine the potential of Quercetin (QE) to protect INS-1 cells from the H2O2-induced oxidative stress and the effects of QE on the glucose-stimulated insulin secretion in INS-1 cells.
Methods: To study the cell viability, cells were incubated with H2O2 and/or QE at the various concentrations. To confirm the protective effect by QE in response to H2O2, the levels of antioxidant enzymes were assessed by RT-PCR and Western blot, and glutathione peroxidase activities were quantified by spectrophotometrical method. Glucose-stimulated insulin secretion (GSIS) was measured by ELISA.
Results: Cell incubations were performed with 80 microM of H2O2 for 5 hours to induce 40 - 50% of cell death. QE gradually showed protective effect (IC50 = 50 microM) in dose-dependent manner. Superoxide dismutase (SOD) mRNA level in H2O2 + QE group was increased as compared to H2O2 group, but catalase did not changed. And the QE recruited glutathione peroxidase activity against H2O2-induced oxidative injuries in INS-1 cells.
Conclusion: In conclusion, these findings suggest that QE might have protective effect on beta cells by ameliorating oxidative stress and preserving insulin secretory function.
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KEYWORD
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Antioxidant effect, Diabetes mellitus, INS-1 cell, Oxidative stress, Quercetin
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